Aisha Huang, a citizen of China, entered a not guilty plea to two charges on Wednesday at the Circuit Court in Accra.
The charges include mining without a license and selling and buying minerals without a license.
Three other people also showed up, including Aisha Huang, who spoke through an interpreter.
The Accra Circuit Court 9 denied galamsey queen, Aisha Huang, bail.
Aisha Huang is currently standing trial with three other Chinese nationals; Jong Li Hua, Huang Jei and Huaid Hai Hun.
They have all been charged with mining without a valid license and engaging in the sale and purchase of minerals without a permit.
Since Aisha Huang was arrested earlier in September, there has been controversy about how she left Ghana and how she ended up back in the country.
President Akufo-Addo has joined the calls for Aisha Huang’s prosecution in the meanwhile after she was detained once more for taking part in illicit mining in the nation.
The infamous galamsey Queen was initially detained in May 2017 and accused of engaging in illegal mining, but she was later deported.
She was, however, once again detained by the National Security in Kumasi two weeks ago for allegedly slipping back into the nation to carry on with her galamsey business, which infuriated Ghanaians who are now demanding her prosecution.
But as part of a two-day visit to the Volta region, President Akufo-Addo spoke at a meeting of the Ghana Bar Association in Ho and expressed support for the Attorney General’s prosecution of the suspect.
“to show you of my full support of the Attorney General in his determination to prosecute Aisha Huang and her collaborators who apparently insist on flouting our laws on galamsey and illegal mining.”
President Akufo-Addo emphasized, “I expect if they are found guilty, that the court would use the full rigor of the new modified Act 995 which has greatly raised the punishment for breaches of the law.